BLASTP 2.2.1 [Apr-13-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|15645712|ref|NP_207889.1| conserved hypothetical
secreted protein [Helicobacter pylori 26695]
(290 letters)
Database: /var/www/html/HP/blast_new/blast/db/pdbaa
13,198 sequences; 2,899,336 total letters
Searching...........................done
Score E
Sequences producing significant alignments: (bits) Value
pdb|1KLX|A Chain A, Helicobacter Pylori Cysteine Rich Prote... 117 2e-27
pdb|1ELR|A Chain A, Crystal Structure Of The Tpr2a-Domain O... 30 0.25
pdb|1KW2|A Chain A, Crystal Structure Of Uncomplexed Vitami... 28 1.2
pdb|1LOT|A Chain A, Crystal Structure Of The Complex Of Act... 28 1.2
pdb|1AI2| Isocitrate Dehydrogenase Complexed With Isocitr... 27 2.1
pdb|1IDC| Isocitrate Dehydrogenase From E.Coli (Mutant K2... 27 2.1
pdb|4ICD| Phosphorylated Isocitrate Dehydrogenase (E.C.1.... 27 2.1
pdb|1CW7|A Chain A, Low Temperature Structure Of Wild-Type ... 27 2.1
pdb|6ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) (Mutant... 27 2.1
pdb|1ISO| Isocitrate Dehydrogenase: Structure Of An Engin... 27 2.1
pdb|1BL5| Isocitrate Dehydrogenase From E. Coli Single Tu... 27 2.1
pdb|1GRO| Oxidoreductase, Nadp, Phosphorylation, Glyoxyla... 27 2.1
pdb|1GRP| Oxidoreductase, Nadp, Phosphorylation, Glyoxyla... 27 2.1
pdb|1HJ6|A Chain A, Isocitrate Dehydrogenase S113e Mutant C... 27 2.1
pdb|1IDE| Isocitrate Dehydrogenase Y160f Mutant Steady-St... 27 2.1
pdb|2TOD|A Chain A, Ornithine Decarboxylase From Trypanosom... 26 4.7
pdb|1JXL|A Chain A, Crystal Structure Of A Y-Family Dna Pol... 26 6.1
pdb|1LH0|A Chain A, Crystal Structure Of Salmonella Typhimu... 26 6.1
>pdb|1KLX|A Chain A, Helicobacter Pylori Cysteine Rich Protein B (Hcpb)
Length = 138
Score = 117 bits (293), Expect = 2e-27
Identities = 61/136 (44%), Positives = 80/136 (57%), Gaps = 8/136 (5%)
Query: 74 GQGVEKNLKKAASFYAKACDLNYSNGC-HLLGNLYYSGQGVSQNTNKALQYYSKACDLKY 132
G V+K+LKKA +Y KAC+LN GC L+ N N K QY SKAC+L
Sbjct: 4 GGTVKKDLKKAIQYYVKACELNEMFGCLSLVSNSQI-------NKQKLFQYLSKACELNS 56
Query: 133 AEGCASLGGIYHDGKVVTRDFKKAVEYFTKACDLNDGDGCTILGSLYDAGRGTPKDLKKA 192
GC LG Y +GK V +D +KA +Y++KAC LND DGC ILG AG+G K+ K+A
Sbjct: 57 GNGCRFLGDFYENGKYVKKDLRKAAQYYSKACGLNDQDGCLILGYKQYAGKGVVKNEKQA 116
Query: 193 LASYDKACDLKDSPGC 208
+ +++KAC L C
Sbjct: 117 VKTFEKACRLGSEDAC 132
Score = 114 bits (284), Expect = 2e-26
Identities = 57/138 (41%), Positives = 81/138 (58%), Gaps = 6/138 (4%)
Query: 146 GKVVTRDFKKAVEYFTKACDLNDGDGCTILGSLYDAGRGTPKDLKKALASYDKACDLKDS 205
G V +D KKA++Y+ KAC+LN+ GC L S + +K KAC+L
Sbjct: 4 GGTVKKDLKKAIQYYVKACELNEMFGCLSLVSNSQINK------QKLFQYLSKACELNSG 57
Query: 206 PGCFNAGNMYHHGEGATKNFKEALARYSKACELENGGGCFNLGAMQYNGEGVTRNEKQAI 265
GC G+ Y +G+ K+ ++A YSKAC L + GC LG QY G+GV +NEKQA+
Sbjct: 58 NGCRFLGDFYENGKYVKKDLRKAAQYYSKACGLNDQDGCLILGYKQYAGKGVVKNEKQAV 117
Query: 266 ENFKKGCKLGAKGACDIL 283
+ F+K C+LG++ AC IL
Sbjct: 118 KTFEKACRLGSEDACGIL 135
Score = 106 bits (264), Expect = 4e-24
Identities = 56/136 (41%), Positives = 79/136 (57%), Gaps = 6/136 (4%)
Query: 42 EKDFTQAKKYFEKACDLKENSGCFNLGVLYYQGQGVEKNLKKAASFYAKACDLNYSNGCH 101
+KD +A +Y+ KAC+L E GC +L + N +K + +KAC+LN NGC
Sbjct: 8 KKDLKKAIQYYVKACELNEMFGCLSLV------SNSQINKQKLFQYLSKACELNSGNGCR 61
Query: 102 LLGNLYYSGQGVSQNTNKALQYYSKACDLKYAEGCASLGGIYHDGKVVTRDFKKAVEYFT 161
LG+ Y +G+ V ++ KA QYYSKAC L +GC LG + GK V ++ K+AV+ F
Sbjct: 62 FLGDFYENGKYVKKDLRKAAQYYSKACGLNDQDGCLILGYKQYAGKGVVKNEKQAVKTFE 121
Query: 162 KACDLNDGDGCTILGS 177
KAC L D C IL +
Sbjct: 122 KACRLGSEDACGILNN 137
Score = 62.0 bits (149), Expect = 8e-11
Identities = 28/66 (42%), Positives = 38/66 (57%)
Query: 40 YKEKDFTQAKKYFEKACDLKENSGCFNLGVLYYQGQGVEKNLKKAASFYAKACDLNYSNG 99
Y +KD +A +Y+ KAC L + GC LG Y G+GV KN K+A + KAC L +
Sbjct: 72 YVKKDLRKAAQYYSKACGLNDQDGCLILGYKQYAGKGVVKNEKQAVKTFEKACRLGSEDA 131
Query: 100 CHLLGN 105
C +L N
Sbjct: 132 CGILNN 137
>pdb|1ELR|A Chain A, Crystal Structure Of The Tpr2a-Domain Of Hop In Complex
With The Hsp90-Peptide Meevd
Length = 131
Score = 30.4 bits (67), Expect = 0.25
Identities = 24/104 (23%), Positives = 46/104 (44%), Gaps = 19/104 (18%)
Query: 35 LGAKSYKEKDFTQAKKYFEKACDLKENSGCF--NLGVLYYQGQGVEKNLKKAASFYAKAC 92
LG +YK+KDF A K+++KA +L + + N +Y++ + + K KA
Sbjct: 10 LGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFE----KGDYNKCRELCEKAI 65
Query: 93 DLNYSN---------GCHLLGNLYYSGQGVSQNTNKALQYYSKA 127
++ N +GN Y+ + A+ +Y+K+
Sbjct: 66 EVGRENREDYRQIAKAYARIGNSYFK----EEKYKDAIHFYNKS 105
>pdb|1KW2|A Chain A, Crystal Structure Of Uncomplexed Vitamin D-Binding Protein
pdb|1KW2|B Chain B, Crystal Structure Of Uncomplexed Vitamin D-Binding Protein
pdb|1KXP|D Chain D, Crystal Structure Of Human Vitamin D-Binding Protein In
Complex With Skeletal Actin
Length = 458
Score = 28.1 bits (61), Expect = 1.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNA 211
K L+ L S + CD++DS CFNA
Sbjct: 347 KVLEPTLKSLGECCDVEDSTTCFNA 371
>pdb|1LOT|A Chain A, Crystal Structure Of The Complex Of Actin With Vitamin D-
Binding Protein
pdb|1J7E|B Chain B, A Structural Basis For The Unique Binding Features Of The
Human Vitamin D-Binding Protein
pdb|1J7E|A Chain A, A Structural Basis For The Unique Binding Features Of The
Human Vitamin D-Binding Protein
pdb|1J78|B Chain B, Crystallographic Analysis Of The Human Vitamin D Binding
Protein
pdb|1J78|A Chain A, Crystallographic Analysis Of The Human Vitamin D Binding
Protein
Length = 458
Score = 28.1 bits (61), Expect = 1.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNA 211
K L+ L S + CD++DS CFNA
Sbjct: 347 KVLEPTLKSLGECCDVEDSTTCFNA 371
>pdb|1AI2| Isocitrate Dehydrogenase Complexed With Isocitrate, Nadp+, And
Calcium (Flash-Cooled)
pdb|1AI3| Orbital Steering In The Catalytic Power Of Enzymes: Small
Structural Changes With Large Catalytic Consequences
pdb|1IKA| Isocitrate Dehydrogenase (E.C.1.1.1.42) Complexed With
Alpha-Ketoglutarate
pdb|1SJS| Access To Phosphorylation In Isocitrate Dehydrogenase May Occur By
Domain Shifting
pdb|5ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) Complex With Mg2+ And
Isocitrate
pdb|9ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) Complex With NADP+
pdb|3ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42)
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1IDC| Isocitrate Dehydrogenase From E.Coli (Mutant K230m), Steady-State
Intermediate Complex Determined By Laue Crystallography
pdb|1IDF| Isocitrate Dehydrogenase K230m Mutant Apo Enzyme
pdb|1CW1|A Chain A, Crystal Structure Of Isocitrate Dehydrogenase Mutant K230m
Bound To Isocitrate And Mn2+
pdb|1CW4|A Chain A, Crystal Structure Of K230m Isocitrate Dehydrogenase In
Complex With Alpha- Ketoglutarate
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHMGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|4ICD| Phosphorylated Isocitrate Dehydrogenase (E.C.1.1.1.42)
Length = 414
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 205 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 259
>pdb|1CW7|A Chain A, Low Temperature Structure Of Wild-Type Idh Complexed With
Mg-Isocitrate
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|6ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) (Mutant With Ser 113
Replaced By Asp) (S113D)
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1ISO| Isocitrate Dehydrogenase: Structure Of An Engineered Nadp+ -->
Nad+ Specificity-Reversal Mutant
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1BL5| Isocitrate Dehydrogenase From E. Coli Single Turnover Laue
Structure Of Rate-Limited Product Complex, 10 Msec Time
Resolution
Length = 414
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 205 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 259
>pdb|1GRO| Oxidoreductase, Nadp, Phosphorylation, Glyoxylate Bypass Mol_id:
1; Molecule: Isocitrate Dehydrogenase; Chain: Null; Ec:
1.1.1.42; Engineered: Yes; Mutation: S113e, N115l
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1GRP| Oxidoreductase, Nadp, Phosphorylation, Glyoxylate Bypass Mol_id:
1; Molecule: Isocitrate Dehydrogenase; Chain: Null; Ec:
1.1.1.42; Mutation: N115l
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1HJ6|A Chain A, Isocitrate Dehydrogenase S113e Mutant Complexed With
Isopropylmalate, Nadp+ And Magnesium (Flash-Cooled)
pdb|7ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) (Mutant With Ser 113
Replaced By Glu) (S113E)
pdb|8ICD| Isocitrate Dehydrogenase (E.C.1.1.1.42) (Mutant With Ser 113
Replaced By Glu) (S113E) Complex With Mg2+ And
Isocitrate
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|1IDE| Isocitrate Dehydrogenase Y160f Mutant Steady-State Intermediate
Complex (Laue Determination)
pdb|1IDD| Isocitrate Dehydrogenase Y160f Mutant Apo Enzyme
Length = 416
Score = 27.3 bits (59), Expect = 2.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 187 KDLKKALASYDKACDLKDSPGCFNAGNMYHHGEGATKNFKEALARYSKACELENGG 242
K L +A Y A D +DS + GN+ EGA K++ LAR EL +GG
Sbjct: 207 KRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGG 261
>pdb|2TOD|A Chain A, Ornithine Decarboxylase From Trypanosoma Brucei K69a
Mutant In Complex With Alpha-Difluoromethylornithine
pdb|2TOD|C Chain C, Ornithine Decarboxylase From Trypanosoma Brucei K69a
Mutant In Complex With Alpha-Difluoromethylornithine
pdb|2TOD|D Chain D, Ornithine Decarboxylase From Trypanosoma Brucei K69a
Mutant In Complex With Alpha-Difluoromethylornithine
pdb|2TOD|B Chain B, Ornithine Decarboxylase From Trypanosoma Brucei K69a
Mutant In Complex With Alpha-Difluoromethylornithine
Length = 425
Score = 26.2 bits (56), Expect = 4.7
Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 4/74 (5%)
Query: 49 KKYFEKACDLKENSGCFNLGVLYYQGQGVEKNLKKAASFYAKACDLNYSNGCHLLGNLYY 108
KK CD + +LG + + + +K L + FYA AC+ ++ +LG L
Sbjct: 27 KKISMNTCDEGDPFFVADLGDIVRKHETWKKCLPRVTPFYAVACNDDW----RVLGTLAA 82
Query: 109 SGQGVSQNTNKALQ 122
G G +N +Q
Sbjct: 83 LGTGFDCASNTEIQ 96
>pdb|1JXL|A Chain A, Crystal Structure Of A Y-Family Dna Polymerase In A
Ternary Complex With Dna Substrates And An Incoming
Nucleotide
Length = 352
Score = 25.8 bits (55), Expect = 6.1
Identities = 13/43 (30%), Positives = 20/43 (46%)
Query: 20 LGGLMAEQDPKELVGLGAKSYKEKDFTQAKKYFEKACDLKENS 62
L G++ E K L+ L Y E T+ +K + +K NS
Sbjct: 213 LKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVTMKRNS 255
>pdb|1LH0|A Chain A, Crystal Structure Of Salmonella Typhimurium Omp Synthase
In Complex With Mgprpp And Orotate
pdb|1LH0|B Chain B, Crystal Structure Of Salmonella Typhimurium Omp Synthase
In Complex With Mgprpp And Orotate
pdb|1OPR| Mol_id: 1; Molecule: Orotate Phosphoribosyltransferase; Chain:
Null; Synonym: Oprtase; Ec: 2.4.2.10; Engineered: Yes
Length = 213
Score = 25.8 bits (55), Expect = 6.1
Identities = 24/80 (30%), Positives = 30/80 (37%), Gaps = 18/80 (22%)
Query: 159 YFTKACDLNDGDGCTILGSLY-----DAG-----------RGTPKDLKKALASYDKACDL 202
YF A N G +LG Y D+G +G P A+A +
Sbjct: 33 YFFNAGLFNTGRDLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHD-- 90
Query: 203 KDSPGCFNAGNMYHHGEGAT 222
KD P CFN HGEG +
Sbjct: 91 KDLPYCFNRKEAKDHGEGGS 110
Database: /var/www/html/HP/blast_new/blast/db/pdbaa
Posted date: Dec 20, 2002 11:08 AM
Number of letters in database: 2,899,336
Number of sequences in database: 13,198
Lambda K H
0.317 0.137 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,904,125
Number of Sequences: 13198
Number of extensions: 88642
Number of successful extensions: 259
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 238
Number of HSP's gapped (non-prelim): 24
length of query: 290
length of database: 2,899,336
effective HSP length: 87
effective length of query: 203
effective length of database: 1,751,110
effective search space: 355475330
effective search space used: 355475330
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)