BLASTP 2.2.1 [Apr-13-2001]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|15644957|ref|NP_207127.1| NH(3)-dependent NAD+
synthetase (nadE) [Helicobacter pylori 26695]
         (260 letters)

Database: /var/www/html/HP/blast_new/blast/db/pdbaa
           13,198 sequences; 2,899,336 total letters

Searching...........................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pdb|1KQP|A  Chain A, Nh3-Dependent Nad+ Synthetase From Baci...    80  2e-16
pdb|1IH8|A  Chain A, Nh3-Dependent Nad+ Synthetase From Baci...    80  2e-16
pdb|1GPM|A  Chain A, Escherichia Coli Gmp Synthetase Complex...    32  0.056
pdb|1G0U|I  Chain I, A Gated Channel Into The Proteasome Cor...    32  0.096
pdb|1RYP|J  Chain J, Crystal Structure Of The 20s Proteasome...    32  0.096
pdb|1CT9|A  Chain A, Crystal Structure Of Asparagine Synthet...    27  2.4
pdb|1J5S|A  Chain A, Crystal Structure Of Uronate Isomerase ...    27  3.1
pdb|1HSJ|A  Chain A, Sarr Mbp Fusion Structure >gi|14488507|...    25  6.9
pdb|1M1X|A  Chain A, Crystal Structure Of The Extracellular ...    25  6.9
pdb|2GBP|    D-GalactoseD-Glucose Binding Protein (GGBP) >gi...    25  9.0
pdb|1C7N|A  Chain A, Crystal Structure Of Cystalysin From Tr...    25  9.0
pdb|3GBP|    Galactose-Binding Protein Complex With Glucose        25  9.0
pdb|1EJ6|C  Chain C, Reovirus Core >gi|9955258|pdb|1EJ6|B Ch...    25  9.0
pdb|1GCA|    GlucoseGALACTOSE-Binding Protein Complex With G...    25  9.0
>pdb|1KQP|A Chain A, Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis At 1
           A Resolution
 pdb|1KQP|B Chain B, Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis At 1
           A Resolution
          Length = 271

 Score = 80.1 bits (196), Expect = 2e-16
 Identities = 69/215 (32%), Positives = 104/215 (48%), Gaps = 19/215 (8%)

Query: 14  DFLEKEVQKRGFKKVVYGLSGGLDSAVVGVLCQ----KVFKENAHALLMPSSVSMP---E 66
           +FL++ V+K G K  V G+SGG DS + G L Q     + +E   A  +  +V +P   +
Sbjct: 27  NFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQFI--AVRLPHGTQ 84

Query: 67  NKTDALNLCEKFSIPYT--EYSIAPYDAIFSSHFKDAS---LT--RKGNFCARLRMAFLY 119
              D   L  KF  P    ++ I    + FS  ++  +   LT   KGN  AR RM   Y
Sbjct: 85  QDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQY 144

Query: 120 DYSLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELARRLNIPKKILN 179
               +   LV+GT + +E + G+ T +GD    + P+  L K +   L + L  P+++  
Sbjct: 145 AIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPERLYL 204

Query: 180 KPPSADLF---VGQSDEKDLGYPYSVIDPLLKDIE 211
           K P+ADL      QSDE +LG  Y  ID  L+  E
Sbjct: 205 KEPTADLLDEKPQQSDETELGISYDEIDDYLEGKE 239
>pdb|1IH8|A Chain A, Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis
           Complexed With Amp-Cpp And Mg2+ Ions.
 pdb|2NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis In Complex With Nad-Adenylate
 pdb|2NSY|B Chain B, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis In Complex With Nad-Adenylate
 pdb|1NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis
 pdb|1NSY|B Chain B, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis
 pdb|1EE1|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With One Molecule Atp, Two
           Molecules Deamido-Nad+ And One Mg2+ Ion
 pdb|1FYD|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With One Molecule Amp, One
           Pyrophosphate Ion And One Mg2+ Ion
 pdb|1IH8|B Chain B, Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis
           Complexed With Amp-Cpp And Mg2+ Ions.
 pdb|1EE1|B Chain B, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With One Molecule Atp, Two
           Molecules Deamido-Nad+ And One Mg2+ Ion
 pdb|1FYD|B Chain B, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With One Molecule Amp, One
           Pyrophosphate Ion And One Mg2+ Ion
 pdb|1IFX|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With Two Molecules
           Deamido-Nad
 pdb|1IFX|B Chain B, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis Complexed With Two Molecules
           Deamido-Nad
          Length = 271

 Score = 80.1 bits (196), Expect = 2e-16
 Identities = 69/215 (32%), Positives = 104/215 (48%), Gaps = 19/215 (8%)

Query: 14  DFLEKEVQKRGFKKVVYGLSGGLDSAVVGVLCQ----KVFKENAHALLMPSSVSMP---E 66
           +FL++ V+K G K  V G+SGG DS + G L Q     + +E   A  +  +V +P   +
Sbjct: 27  NFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQFI--AVRLPHGTQ 84

Query: 67  NKTDALNLCEKFSIPYT--EYSIAPYDAIFSSHFKDAS---LT--RKGNFCARLRMAFLY 119
              D   L  KF  P    ++ I    + FS  ++  +   LT   KGN  AR RM   Y
Sbjct: 85  QDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQY 144

Query: 120 DYSLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELARRLNIPKKILN 179
               +   LV+GT + +E + G+ T +GD    + P+  L K +   L + L  P+++  
Sbjct: 145 AIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPERLYL 204

Query: 180 KPPSADLF---VGQSDEKDLGYPYSVIDPLLKDIE 211
           K P+ADL      QSDE +LG  Y  ID  L+  E
Sbjct: 205 KEPTADLLDEKPQQSDETELGISYDEIDDYLEGKE 239
>pdb|1GPM|A Chain A, Escherichia Coli Gmp Synthetase Complexed With Amp And
           Pyrophosphate
 pdb|1GPM|C Chain C, Escherichia Coli Gmp Synthetase Complexed With Amp And
           Pyrophosphate
 pdb|1GPM|B Chain B, Escherichia Coli Gmp Synthetase Complexed With Amp And
           Pyrophosphate
 pdb|1GPM|D Chain D, Escherichia Coli Gmp Synthetase Complexed With Amp And
           Pyrophosphate
          Length = 525

 Score = 32.3 bits (72), Expect = 0.056
 Identities = 36/188 (19%), Positives = 71/188 (37%), Gaps = 40/188 (21%)

Query: 24  GFKKVVYGLSGGLDSAVVGVLCQKVFKENAHALLMPSSVSMPENKTDALNLC-EKFSIPY 82
           G  KV+ GLSGG+DS+V  +L  +   +N   + + + +         L++  + F +  
Sbjct: 226 GDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDMFGDHFGLNI 285

Query: 83  TEYSIAPYDAIFSSHFKDASLTRKGNFCARLRMAFLYDYSLKSDSLVIGTSNKSERMLGY 142
               +   D   S+   +     K     R+ +    + +LK + +         + L  
Sbjct: 286 VH--VPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDV---------KWLAQ 334

Query: 143 GTLFGDLACA----------------------------INPIGELFKTEVYELARRLNIP 174
           GT++ D+  +                            + P+ ELFK EV ++   L +P
Sbjct: 335 GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELGLP 394

Query: 175 KKILNKPP 182
             +L + P
Sbjct: 395 YDMLYRHP 402
>pdb|1G0U|I Chain I, A Gated Channel Into The Proteasome Core Particle
 pdb|1G0U|W Chain W, A Gated Channel Into The Proteasome Core Particle
 pdb|1FNT|J Chain J, Crystal Structure Of The 20s Proteasome From Yeast In
           Complex With The Proteasome Activator Pa26 From
           Trypanosome Brucei At 3.2 Angstroms Resolution
 pdb|1FNT|X Chain X, Crystal Structure Of The 20s Proteasome From Yeast In
           Complex With The Proteasome Activator Pa26 From
           Trypanosome Brucei At 3.2 Angstroms Resolution
          Length = 205

 Score = 31.6 bits (70), Expect = 0.096
 Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 7/78 (8%)

Query: 115 MAFLYDYSLKSDSLVIGTSNKSERMLGYGTLF---GDLACAINPIGELF--KTEVYELAR 169
           +A   D  L S SL  G SNK E++  YG +F     LA  +  + E+F  KT +Y+L  
Sbjct: 21  VAIACDLRLGSQSL--GVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKE 78

Query: 170 RLNIPKKILNKPPSADLF 187
              I  +   +  S+ L+
Sbjct: 79  ERAIEPETFTQLVSSSLY 96
>pdb|1RYP|J Chain J, Crystal Structure Of The 20s Proteasome From Yeast At 2.4
           Angstroms Resolution
 pdb|1RYP|X Chain X, Crystal Structure Of The 20s Proteasome From Yeast At 2.4
           Angstroms Resolution
 pdb|1G65|I Chain I, Crystal Structure Of Epoxomicin:20s Proteasome Reveals A
           Molecular Basis For Selectivity Of
           Alpha,Beta-Epoxyketone Proteasome Inhibitors
 pdb|1G65|W Chain W, Crystal Structure Of Epoxomicin:20s Proteasome Reveals A
           Molecular Basis For Selectivity Of
           Alpha,Beta-Epoxyketone Proteasome Inhibitors
 pdb|1JD2|I Chain I, Crystal Structure Of The Yeast 20s Proteasome:tmc-95a
           Complex: A Non-Covalent Proteasome Inhibitor
 pdb|1JD2|P Chain P, Crystal Structure Of The Yeast 20s Proteasome:tmc-95a
           Complex: A Non-Covalent Proteasome Inhibitor
          Length = 204

 Score = 31.6 bits (70), Expect = 0.096
 Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 7/78 (8%)

Query: 115 MAFLYDYSLKSDSLVIGTSNKSERMLGYGTLF---GDLACAINPIGELF--KTEVYELAR 169
           +A   D  L S SL  G SNK E++  YG +F     LA  +  + E+F  KT +Y+L  
Sbjct: 20  VAIACDLRLGSQSL--GVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKE 77

Query: 170 RLNIPKKILNKPPSADLF 187
              I  +   +  S+ L+
Sbjct: 78  ERAIEPETFTQLVSSSLY 95
>pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B From
           Escherichia Coli
 pdb|1CT9|B Chain B, Crystal Structure Of Asparagine Synthetase B From
           Escherichia Coli
 pdb|1CT9|C Chain C, Crystal Structure Of Asparagine Synthetase B From
           Escherichia Coli
 pdb|1CT9|D Chain D, Crystal Structure Of Asparagine Synthetase B From
           Escherichia Coli
          Length = 553

 Score = 26.9 bits (58), Expect = 2.4
 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 2/34 (5%)

Query: 16  LEKEVQKRGFKKVVYG--LSGGLDSAVVGVLCQK 47
           LE  V+      V YG  LSGGLDS+++  + +K
Sbjct: 215 LEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKK 248
>pdb|1J5S|A Chain A, Crystal Structure Of Uronate Isomerase (Tm0064) From
           Thermotoga Maritima At 2.85 A Resolution
 pdb|1J5S|B Chain B, Crystal Structure Of Uronate Isomerase (Tm0064) From
           Thermotoga Maritima At 2.85 A Resolution
 pdb|1J5S|C Chain C, Crystal Structure Of Uronate Isomerase (Tm0064) From
           Thermotoga Maritima At 2.85 A Resolution
          Length = 463

 Score = 26.6 bits (57), Expect = 3.1
 Identities = 22/78 (28%), Positives = 34/78 (43%), Gaps = 7/78 (8%)

Query: 49  FKENA-----HALLMPSSVSMPENKTDALNLCEKFSIPYTEYSIAPYDAIFSSHFKDASL 103
           FKE+      HALL PS   + EN+  A++         T+  I  Y A     F    +
Sbjct: 234 FKEHGCVASDHALLEPSVYYVDENRARAVHEKAFSGEKLTQDEINDYKAFMMVQF--GKM 291

Query: 104 TRKGNFCARLRMAFLYDY 121
            ++ N+  +L +  L DY
Sbjct: 292 NQETNWVTQLHIGALRDY 309
>pdb|1HSJ|A Chain A, Sarr Mbp Fusion Structure
 pdb|1HSJ|B Chain B, Sarr Mbp Fusion Structure
          Length = 487

 Score = 25.4 bits (54), Expect = 6.9
 Identities = 16/52 (30%), Positives = 22/52 (41%), Gaps = 2/52 (3%)

Query: 210 IEALFQTKPI--DTETLAQLGYDEILVKNITSRIQKNAFKLELPAIAKRFNP 259
           + A FQ K    DT+    L Y+EI + N   R + N    +  A    F P
Sbjct: 383 VNATFQVKKFFRDTKKKFNLNYEEIYILNHILRSESNEISSKEIAKCSEFKP 434
>pdb|1M1X|A Chain A, Crystal Structure Of The Extracellular Segment Of Integrin
           Alpha Vbeta3 Bound To Mn2+
 pdb|1JV2|A Chain A, Crystal Structure Of The Extracellular Segment Of Integrin
           Alphavbeta3
 pdb|1L5G|A Chain A, Crystal Structure Of The Extracellular Segment Of Integrin
           Avb3 In Complex With An Arg-Gly-Asp Ligand
          Length = 957

 Score = 25.4 bits (54), Expect = 6.9
 Identities = 34/116 (29%), Positives = 49/116 (41%), Gaps = 13/116 (11%)

Query: 146 FGDLACAINPIGELFKTEVYELARR---LN-IPKKIL-------NKPPSADLFV-GQSDE 193
           F D+A A    GE  K  VY    R   LN +P +IL       + PPS    + G +D 
Sbjct: 355 FNDIAIAAPYGGEDKKGIVYIFNGRSTGLNAVPSQILEGQWAARSMPPSFGYSMKGATDI 414

Query: 194 KDLGYPYSVIDPLLKDIEALFQTKPIDTETLAQLGYDEILVK-NITSRIQKNAFKL 248
              GYP  ++     D   L++ +P+ T       Y  IL + N T  +   A K+
Sbjct: 415 DKNGYPDLIVGAFGVDRAILYRARPVITVNAGLEVYPSILNQDNKTCSLPGTALKV 470
>pdb|2GBP|   D-GalactoseD-Glucose Binding Protein (GGBP)
 pdb|1GLG|   GalactoseGLUCOSE-Binding Protein Complexed With D-Galactose
          Length = 309

 Score = 25.0 bits (53), Expect = 9.0
 Identities = 15/37 (40%), Positives = 17/37 (45%)

Query: 147 GDLACAINPIGELFKTEVYELARRLNIPKKILNKPPS 183
           G  A AIN +       V E AR  N+P    NK PS
Sbjct: 59  GVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPS 95
>pdb|1C7N|A Chain A, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|B Chain B, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|C Chain C, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|D Chain D, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|E Chain E, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|F Chain F, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|G Chain G, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7N|H Chain H, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal 5'-Phosphate Cofactor
 pdb|1C7O|A Chain A, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|B Chain B, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|C Chain C, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|D Chain D, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|E Chain E, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|F Chain F, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|G Chain G, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
 pdb|1C7O|H Chain H, Crystal Structure Of Cystalysin From Treponema Denticola
           Contains A Pyridoxal
           5'-Phosphate-L-Aminoethoxyvinylglycine Complex
          Length = 399

 Score = 25.0 bits (53), Expect = 9.0
 Identities = 28/90 (31%), Positives = 43/90 (47%), Gaps = 17/90 (18%)

Query: 154 NPIGELFKTEVYELARRLNIPKKILNKPPSADLFVGQSD-EKDL---GYPYSV---IDPL 206
           NP+G ++K +  EL +  +I  K       +DL +   +   DL   GY ++V   ID  
Sbjct: 175 NPVGRVWKKD--ELQKIKDIVLK-------SDLMLWSDEIHFDLIMPGYEHTVFQSIDEQ 225

Query: 207 LKDIEALFQTKPIDTETLAQLGYDEILVKN 236
           L D    F T P  T  +A +G   I++KN
Sbjct: 226 LADKTITF-TAPSKTFNIAGMGMSNIIIKN 254
>pdb|3GBP|   Galactose-Binding Protein Complex With Glucose
          Length = 305

 Score = 25.0 bits (53), Expect = 9.0
 Identities = 15/37 (40%), Positives = 17/37 (45%)

Query: 147 GDLACAINPIGELFKTEVYELARRLNIPKKILNKPPS 183
           G  A AIN +       V E AR  N+P    NK PS
Sbjct: 57  GVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPS 93
>pdb|1EJ6|C Chain C, Reovirus Core
 pdb|1EJ6|B Chain B, Reovirus Core
          Length = 1275

 Score = 25.0 bits (53), Expect = 9.0
 Identities = 15/58 (25%), Positives = 28/58 (47%), Gaps = 7/58 (12%)

Query: 195 DLGYPYSVIDPLLKDIEALFQTKPIDTETLAQLGYDEILVKNITSRIQKNAFKLELPA 252
           ++G   +VI P+L+DI  L Q        ++ L  D  ++ N  S + ++  +   PA
Sbjct: 524 NIGNNATVIQPVLQDISVLLQ-------RISPLQIDPTIISNTMSTVSESTTQTLSPA 574
>pdb|1GCA|   GlucoseGALACTOSE-Binding Protein Complex With Galactose
 pdb|1GCG|   GalactoseGLUCOSE-Binding Protein (Closed, Unliganded Form)
          Length = 309

 Score = 25.0 bits (53), Expect = 9.0
 Identities = 15/37 (40%), Positives = 17/37 (45%)

Query: 147 GDLACAINPIGELFKTEVYELARRLNIPKKILNKPPS 183
           G  A AIN +       V E AR  N+P    NK PS
Sbjct: 59  GVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPS 95
  Database: /var/www/html/HP/blast_new/blast/db/pdbaa
    Posted date:  Dec 20, 2002 11:08 AM
  Number of letters in database: 2,899,336
  Number of sequences in database:  13,198
  
Lambda     K      H
   0.319    0.138    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,469,891
Number of Sequences: 13198
Number of extensions: 59412
Number of successful extensions: 129
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 119
Number of HSP's gapped (non-prelim): 14
length of query: 260
length of database: 2,899,336
effective HSP length: 86
effective length of query: 174
effective length of database: 1,764,308
effective search space: 306989592
effective search space used: 306989592
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)