BLASTP 2.2.1 [Apr-13-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|15645904|ref|NP_208083.1| conserved hypothetical
protein [Helicobacter pylori 26695]
(204 letters)
Database: /home/scwang/download_20020708_db/nr
1,026,957 sequences; 324,149,939 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_208083.1| (NC_000915) conserved hypothetical protein... 405 e-112
ref|NP_223929.1| (NC_000921) putative [Helicobacter pylori ... 377 e-104
ref|NP_562651.1| (NC_003366) conserved hypothetical protein... 83 2e-15
ref|NP_348357.1| (NC_003030) Predicted nucleotide-binding p... 78 5e-14
ref|NP_623111.1| (NC_003869) Thiamine pyrophosphokinase [Th... 72 5e-12
ref|NP_387356.1| (NC_003047) CONSERVED HYPOTHETICAL PROTEIN... 46 3e-04
ref|NP_603787.1| (NC_003454) Thiamin pyrophosphokinase [Fus... 43 0.002
ref|NP_593291.1| (NC_003424) thiamine pyrophosphokinase [Sc... 42 0.003
>ref|NP_208083.1| (NC_000915) conserved hypothetical protein [Helicobacter pylori
26695]
pir||C64681 conserved hypothetical protein HP1291 - Helicobacter pylori
(strain 26695)
gb|AAD08334.1| (AE000633) conserved hypothetical protein [Helicobacter pylori
26695]
Length = 204
Score = 405 bits (1041), Expect = e-112
Identities = 204/204 (100%), Positives = 204/204 (100%)
Query: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA
Sbjct: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
Query: 61 LYNPIRMSEQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYFKFCQKI 120
LYNPIRMSEQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYFKFCQKI
Sbjct: 61 LYNPIRMSEQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYFKFCQKI 120
Query: 121 QAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKAQFTSKNLKYPLKNLRLKTLFSGSLN 180
QAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKAQFTSKNLKYPLKNLRLKTLFSGSLN
Sbjct: 121 QAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKAQFTSKNLKYPLKNLRLKTLFSGSLN 180
Query: 181 EATDSYFSLSSTPKSVVLVYQKFL 204
EATDSYFSLSSTPKSVVLVYQKFL
Sbjct: 181 EATDSYFSLSSTPKSVVLVYQKFL 204
>ref|NP_223929.1| (NC_000921) putative [Helicobacter pylori J99]
pir||C71832 hypothetical protein jhp1211 - Helicobacter pylori (strain J99)
gb|AAD06813.1| (AE001547) putative [Helicobacter pylori J99]
Length = 204
Score = 377 bits (967), Expect = e-104
Identities = 187/203 (92%), Positives = 199/203 (97%)
Query: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
MQAVILANGEFPKS+KCLD+L+NAPFLIACDGAV SLHALQFKPSVVIGDLDSIDSHLKA
Sbjct: 1 MQAVILANGEFPKSKKCLDILQNAPFLIACDGAVISLHALQFKPSVVIGDLDSIDSHLKA 60
Query: 61 LYNPIRMSEQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYFKFCQKI 120
LYNPIR+SEQ+SNDLSKAFFYALN+GCDDFIFLGLNGKREDHALANTFLLLEYFKFC+KI
Sbjct: 61 LYNPIRVSEQDSNDLSKAFFYALNRGCDDFIFLGLNGKREDHALANTFLLLEYFKFCKKI 120
Query: 121 QAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKAQFTSKNLKYPLKNLRLKTLFSGSLN 180
Q++SDYGLFRVLETPFTLPSFKGEQISLFSLDLKA+FTSKNLKYPLK+LRLKTLFSGSLN
Sbjct: 121 QSVSDYGLFRVLETPFTLPSFKGEQISLFSLDLKARFTSKNLKYPLKDLRLKTLFSGSLN 180
Query: 181 EATDSYFSLSSTPKSVVLVYQKF 203
EAT+ FSLSS PKSVVLVYQKF
Sbjct: 181 EATNHCFSLSSEPKSVVLVYQKF 203
>ref|NP_562651.1| (NC_003366) conserved hypothetical protein [Clostridium
perfringens]
dbj|BAB81441.1| (AP003191) conserved hypothetical protein [Clostridium perfringens]
Length = 209
Score = 83.2 bits (204), Expect = 2e-15
Identities = 63/210 (30%), Positives = 95/210 (45%), Gaps = 10/210 (4%)
Query: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
M+ +I+A GE P S+ +K LIA D + F P +GD DSI K
Sbjct: 1 MKVLIIAGGEKPSSELLKSEIKECDLLIAADRGAEAYLENGFTPDYALGDFDSIGEEYKD 60
Query: 61 LYNPIRMS----EQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYFKF 116
+ + I++ E+++ D AFF A+ G + +FLG+ G R DH +AN LL E +
Sbjct: 61 ILDKIKVEKFNPEKDNTDTEIAFFKAVELGATEIVFLGVTGTRLDHVMANLGLLREALER 120
Query: 117 CQKIQAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKA-QFTSKNLKYPLKNLRLKTLF 175
+ I + + TL G+ IS + F+ K KY L N +L L
Sbjct: 121 GIEAYIIDNNNKIYLKNKETTLKKEFGDYISFQAFGSPVNNFSIKGSKYELYNHKL--LI 178
Query: 176 SGSL---NEATDSYFSLSSTPKSVVLVYQK 202
SL NE D Y +S V+++Y +
Sbjct: 179 GDSLCVSNEFNDEYIDISFEKGEVLVIYSR 208
>ref|NP_348357.1| (NC_003030) Predicted nucleotide-binding protein, YLOS B.subtilis
ortholog [Clostridium acetobutylicum]
gb|AAK79697.1|AE007682_7 (AE007682) Predicted nucleotide-binding protein, YLOS B.subtilis
ortholog [Clostridium acetobutylicum]
Length = 211
Score = 78.2 bits (191), Expect = 5e-14
Identities = 53/208 (25%), Positives = 96/208 (45%), Gaps = 10/208 (4%)
Query: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
M+ V+++ G+ P ++N ++I D LH + +P +++GD DSID +
Sbjct: 1 MKVVVISGGKMPSEALLRSEIENCDYIICADSGANCLHKYEIRPDMLLGDFDSIDEEVFN 60
Query: 61 LYNPIRMS------EQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYF 114
+ ++ E++ D AF AL D+ FLG G R DH N LL
Sbjct: 61 YFKEFHINTIKFPREKDFTDTELAFREALKLSADEICFLGCTGTRLDHIFGNLGLLYRCL 120
Query: 115 KFCQKIQAISDYGLFRVLETPFTLPSFKGEQISL--FSLDLKAQFTSKNLKYPLKNLRLK 172
K + D +++ ++ KGE S+ F ++K + + +N KYPLK+ L
Sbjct: 121 KSGIRAYIKDDNNTLFMIDKTISITGKKGEIFSIQGFREEIK-ELSIENAKYPLKDYNLS 179
Query: 173 TLFSGSL-NEATDSYFSLSSTPKSVVLV 199
S ++ NE D ++S +++++
Sbjct: 180 FGDSRTVSNEFLDEPVTISFKNGTIIVM 207
>ref|NP_623111.1| (NC_003869) Thiamine pyrophosphokinase [Thermoanaerobacter
tengcongensis]
gb|AAM24715.1| (AE013107) Thiamine pyrophosphokinase [Thermoanaerobacter
tengcongensis]
Length = 211
Score = 71.6 bits (174), Expect = 5e-12
Identities = 48/174 (27%), Positives = 87/174 (49%), Gaps = 9/174 (5%)
Query: 1 MQAVILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
M+ +I++NGE ++++ +I DG + ++ +P ++IGD DS+D +
Sbjct: 1 MKVLIISNGEIKDYGFYKNIVEEVDMVICADGGANHAYKMKIRPFLIIGDFDSVDREVLE 60
Query: 61 LYNP--IRMSE----QNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYF 114
Y +++ + ++ D A A+ G D F+G+ G R DH+ AN LLL
Sbjct: 61 FYQKEGVKVEKFPTMKDETDTQLALKKAIELGAKDITFIGVIGDRFDHSYANLSLLLYSL 120
Query: 115 KFCQKIQAISDYGLFRVLETPFTLPSFKGEQISL--FSLDLKAQFTSKNLKYPL 166
K K + I++ +++ + KGE +SL +S ++K +T K L YPL
Sbjct: 121 KRGVKSRIINEKNEIHLIDDYIEIEGKKGELLSLLPYSGEVKGIYT-KGLFYPL 173
>ref|NP_387356.1| (NC_003047) CONSERVED HYPOTHETICAL PROTEIN [Sinorhizobium meliloti]
emb|CAC47829.1| (AL591793) CONSERVED HYPOTHETICAL PROTEIN [Sinorhizobium meliloti]
Length = 216
Score = 45.8 bits (107), Expect = 3e-04
Identities = 47/188 (25%), Positives = 76/188 (40%), Gaps = 11/188 (5%)
Query: 5 ILANGEFPKSQKCLDLLKNAPFLIACDGAVTSLHALQFKPSVVIGDLDSIDSHLKALYNP 64
IL G + + L+ + F +A DG + L P + +GD DS D L A +
Sbjct: 8 ILLGGALTPTDRLARQLEGSRF-VAADGGMRHARTLGVVPDLWVGDFDSTDEALLAEFAG 66
Query: 65 I----RMSEQNSNDLSKAFFYALNKGCDDFIFL-GLNGKREDHA---LANTFLLLEYFKF 116
+ + +N+ D A AL +G +F GL G R DHA L T L E
Sbjct: 67 VPRERHPAAKNATDGEIAVEAALERGATSLVFAGGLGGARSDHAFLHLLGTVALAE--SG 124
Query: 117 CQKIQAISDYGLFRVLETPFTLPSFKGEQISLFSLDLKAQFTSKNLKYPLKNLRLKTLFS 176
+ + + +L + KG S+ + +N++YPLK+ L S
Sbjct: 125 VAVMMTSGEEEAYPLLPGSQEIELPKGSLFSILGFADLDGLSIENVRYPLKDFHLPFGSS 184
Query: 177 GSLNEATD 184
+++ D
Sbjct: 185 RTISNIAD 192
>ref|NP_603787.1| (NC_003454) Thiamin pyrophosphokinase [Fusobacterium nucleatum
subsp. nucleatum ATCC 25586]
gb|AAL95086.1| (AE010597) Thiamin pyrophosphokinase [Fusobacterium nucleatum
subsp. nucleatum ATCC 25586]
Length = 209
Score = 43.1 bits (100), Expect = 0.002
Identities = 50/180 (27%), Positives = 73/180 (39%), Gaps = 16/180 (8%)
Query: 3 AVILANGEFPKSQKC-LDLLKNAPFLIAC-DGAVTSLHALQFKPSVVIGDLDSIDSHLKA 60
A + NGE + LD +KN I C DG + L P + GDLDSI +K
Sbjct: 4 AYLFLNGELRGDKNFYLDFIKNHKGDIYCADGGANFCYELTLIPKEIYGDLDSIKDEVKE 63
Query: 61 LYNPIRMS------EQNSNDLSKAFFYALNKGCDDFIFLGLNGKREDHALANTFLLLEYF 114
Y ++ E++ D NK + GL G DH L N LL +Y
Sbjct: 64 FYQEKKVKFIKFKIEKDYTDSELLLNEIQNKYDVIYCIAGLGGS-IDHELTNINLLAKY- 121
Query: 115 KFCQKIQAISDYGLFRVLETPFTLPSFKGEQIS--LFSLDLKAQFTSKNLKYPLKNLRLK 172
+ IS+ +++ +IS +FS +K T K KY ++NL +K
Sbjct: 122 ---SNLIFISEKEKIFKIDSDSKFNDMINTKISFVIFSDQVKG-LTLKGFKYSIENLDIK 177
>ref|NP_593291.1| (NC_003424) thiamine pyrophosphokinase [Schizosaccharomyces pombe]
sp|P41888|TNR3_SCHPO Thiamine pyrophosphokinase (TPK) (Thiamine kinase)
pir||S52350 thiamin pyrophosphokinase (EC 2.7.6.2) - fission yeast
(Schizosaccharomyces pombe)
emb|CAA59135.1| (X84417) thiamin pyrophosphokinase [Schizosaccharomyces pombe]
emb|CAB11089.1| (Z98533) thiamine pyrophosphokinase [Schizosaccharomyces pombe]
Length = 569
Score = 42.4 bits (98), Expect = 0.003
Identities = 29/94 (30%), Positives = 38/94 (39%), Gaps = 7/94 (7%)
Query: 20 LLKNAPFLIACDGAVTSL--HALQFKPSVVIGDLDSIDSHLKALYNPIRMSE-----QNS 72
L K A + DG L + KP V+GD DS+ KA Y + ++ QN+
Sbjct: 372 LWKRASIRVCADGGANQLRNYDSSLKPDYVVGDFDSLTDETKAYYKEMGVNIVFDPCQNT 431
Query: 73 NDLSKAFFYALNKGCDDFIFLGLNGKREDHALAN 106
D K G D L G R DHA+ N
Sbjct: 432 TDFMKCHKIIKEHGIDTIFVLCGMGGRVDHAIGN 465
Database: /home/scwang/download_20020708_db/nr
Posted date: Aug 7, 2002 12:55 PM
Number of letters in database: 324,149,939
Number of sequences in database: 1,026,957
Lambda K H
0.322 0.138 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,497,872
Number of Sequences: 1026957
Number of extensions: 4743021
Number of successful extensions: 9088
Number of sequences better than 1.0e-02: 8
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 9079
Number of HSP's gapped (non-prelim): 8
length of query: 204
length of database: 324,149,939
effective HSP length: 115
effective length of query: 89
effective length of database: 206,049,884
effective search space: 18338439676
effective search space used: 18338439676
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 94 (40.8 bits)